Fructosamine assay modified for the estimation of glycated hemoglobin.
نویسندگان
چکیده
CLINICAL CHEMISTRY, Vol. 35, No. 3, 1989 497 4#{176}C. For 12 additional patients (group B) the analysis was performed immediately and repeated 10 and 30 days after collection of the samples, which were stored at room temperature in airtight containers. The data were examined by Student’s paired t-test and linear-regression analysis. In neither group did storage conditions significantly influence the SCN measured two months (group A, t = 0.569, NS), 10 days (group B, t = 0.419, NS), or 30 days (group B, t = 0.01, NS) after sampling. Results of prompt and postponed analysis correlated well (Figure 1). In group B, culture of the samples confirmed in all cases the presence of polymicrobial flora normally present in oropharynx. We conclude that SCN is stable in saliva stored in airtight containers for as long as one month at room temperature or two months at 4 #{176}C-an dditional advantage for the largescale application of the test in epidemiological studies.
منابع مشابه
Fructosamine and glycated hemoglobin in the assessment of glycaemic control in dogs.
Fructosamine and glycated hemoglobin (HbA1c) concentrations were measured simultaneously in 222 dogs (96 healthy and 126 sick dogs). The dogs were divided into 3 groups according to the glucose concentration: hypo, hyper and euglycaemic dogs. Serum fructosamine concentrations were measured by the reduction test with nitroblue tetrazolium. A turbidimetric inhibition immunoassay and specific poly...
متن کاملComparative study of NBT reduction method for estimation of glycated protein (serum fructoseamine) with glycated HbA1c estimated on DCA 2000+Analyzer (immunoagglutination inhibition).
Glycated protein estimation is a diagnostic tool, used for the long term and short term monitoring of the glycemic status of diabetic patients. The present study is designed to compare and correlate modified NBT reduction method for the estimation of Glycated protein (serum fructosamine) with HbAlc estimated on DCA+2000 Analyzer. Glycated protein (serum fructosamine) reduces Nitro Blue Tetrazol...
متن کاملSerum fructosamine and glycated haemoglobin measurements in diabetic control.
Serum fructosamine and glycated haemoglobin (HbA1) were measured in capillary samples from diabetic children and compared with samples from non-diabetic children. Glycaemic control was assessed clinically and by average daily glucose values recorded by home monitoring. Fructosamine correlated with HbA1 and with average glucose values measured over 30 days. HbA1 also correlated with average gluc...
متن کاملFructosamine: structure, analysis, and clinical usefulness.
Glucose molecules are joined to protein molecules to form stable ketoamines, or fructosamines, through glycation, a nonenzymatic mechanism involving a labile Schiff base intermediate and the Amadori rearrangement. The amount of fructosamine in serum is increased in diabetes mellitus owing to the abnormally high concentration of sugar in blood. The concentration of fructosamine in serum thus ref...
متن کاملGlycated calmodulin from platelets as an index of glycemic control.
In an effort to test whether a significant fraction of calmodulin would become glycated within the life span of the platelet (10-14 days), we monitored the kinetics of calmodulin glycation in vitro. Under the conditions we used, the fraction of glycated calmodulin reached a maximum (approximately 21%) within 10 days. We then extended the studies to human subjects. The intraplatelet concentratio...
متن کاملLaboratory monitoring of gestational diabetes.
The consequences of uncontrolled gestational diabetes is severe to both maternal and fetal well-being. An ideal laboratory test to monitor gestational diabetes should accurately reflect short-term glucose changes. Glycated albumin, by virtue of its short half-life of 14 to 19 days, lends itself as a test to monitor and control gestational diabetes. Analytical approaches for the measurement of g...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 35 3 شماره
صفحات -
تاریخ انتشار 1989